NLRP3 Rabbit Polyclonal Antibody

SKU: orb1294254

Description

Rabbit polyclonal antibody to NLRP3

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 3

Tested Applications	IF, IHC, WB
Dilution Range	Western Blot 1:500-1:1000, Immunofluorescence 1:200–1:300, Immunohistochemistry (Paraffin) 1:100 – 1:200
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide / encompassing a sequence within the N-terminus region.
Target	NLRP3
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Anti-NLRP3 antibody - at 1/700 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294254 overnight at 4°C.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294254 anti-NLRP3 antibody (red) at 1:200 and 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling NLRP3 at 1/100.

Quick Database Links

Gene Symbol

NLRP3

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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