NOX2 Recombinant Rabbit Monoclonal Antibody

SKU: orb1499387

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Description

NOX2 Recombinant Rabbit Monoclonal Antibody

Research Area

Metabolism Research

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution Range	WB=1:1000-5000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Biotin

HRP

IRDye800CW

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	B1D34
Immunogen	A synthesized peptide derived from human NOX2 (142-204/570aa)
Target	CYBB
Molecular Weight	55 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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NOX2; AMCBX2; CGD; CGDX; GP91-1; GP91-PHOX; GP91PHOX; IMD34; p91-PHOX; CGD91-phox; Cyd; CY24B_BOVIN; CYBB; Cytochrome b(558) subunit beta (Cytochrome b558 subunit beta); Cytochrome b-245 heavy chain; Heme-binding membrane glycoprotein gp91phox; Neutrophil cytochrome b 91 kDa polypeptide; p22 phagocyte B-cytochrome; 1.6.3.-; CY24B_HUMAN; Superoxide-generating NADPH oxidase heavy chain subunit; CY24B_MOUSE;

Quality Guarantee

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NOX2 Recombinant Rabbit Monoclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HepG2, 2: MCF-7, 3: Mouse spleen, 4: Rat spleen, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 65 kDa, Observed MW: 60 kDa.

Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug, Lane 2: Hela (Human) Cell Lysate at 30 ug, Lane 3: HepG2 (Human) Cell Lysate at 30 ug, Lane 4: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-NOX2 (orb1499387) at 1/1000 dilution, Anti-beta-Actin (orb500817) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58-65 kD, Observed band size: 58 kD.

Quick Database Links

Gene Symbol

CYBB

UniProt

P04839

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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