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NQO1 Recombinant Rabbit Monoclonal Antibody
SKU: orb612235
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Description
Images & Validation
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| Tested Applications | FC, ICC, WB |
|---|---|
| Dilution range | WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1:50-100 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 8T5 |
| Immunogen | A synthesized peptide derived from human NQO1 (1-46/274aa) |
| Target | NQO1 |
| Molecular Weight | 31 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Azoreductase; Cytochrome b 5 reductase; DHQU; DIA 4; DIA4; Diaphorase(NADH/NADPH)(cytochrome b 5 reductase); Diaphorase(NADH/NADPH)(cytochrome b-5 reductase); Diaphorase(NADH/NADPH)(cytochrome b-5 reductase); Diaphorase(NADH/NADPH); Diaphorase 4; Dioxin inducible 1; DT diaphorase; DT-diaphorase; DTD; Menadione reductase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase quinone 1; NAD(P)H menadione oxidoreductase 1 dioxin inducible; NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NMOR 1; NMOR I; NMOR1; NMORI; NQO 1; NQO1_HUMAN; Phylloquinone reductase; Phylloquinone reductase; QR 1; QR1; Quinone reductase 1; Quinone reductase 1.
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Blank control: Hela. Primary Antibody (green line): Rabbit Anti-NQO1 antibody (orb612235), dilution: 1:100, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1:1000. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Sample: Lane 1: mouse kidney tissue lysate, Lane 2: SH-SY5Y cell lysate, Primary: Anti-NQO1 (orb612235) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 31 kD, Observed band size: 31 kD.
Quick Database Links
Gene Symbol
NQO1
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ICC
Immunocytochemistry
NQO1 Recombinant Rabbit Monoclonal Antibody (orb612235)
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