NR5A2/LRH-1 Antibody

SKU: orb1533776

This product can be custom made

Description

Research Area

Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Tested Applications	IHC, IHC-P
Dilution Range	IHC, IHC-P (10 - 20 µg/ml)
Reactivity	Canine, Equine, Gallus, Hamster, Human, Monkey, Mouse, Rat, Xenopus, Zebrafish
Application Notes	Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 2.5 - 5 ug/ml. Antibody does not work with tissues fixed in 4% PFA. Read more...

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic 16 amino acid peptide from internal region of human NR5A2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Hamster, Elephant, Panda, Horse, Opossum, Turkey, Chicken, Platypus, Xenopus, Trout, Zebrafish, Stickleback (100%); Sheep, Bovine, Bat, Pufferfish (94%); Chimpanzee (88%); Catfish (81%).
Target	NR5A2 / LRH-1
Purification	Immunoaffinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS, 0.1% Sodium Azide
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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NR5A2, B1F, B1F2, B1-binding factor, CPF, CYP7A promoter-binding factor, FTZ-F1beta, FTF, Liver receptor homolog 1, LRH1, LRH-1, HB1F, HB1F-2, Nuclear receptor phr-1, FTZ-F1, Liver receptor homolog-1, Nuclear receptor NR5A2, Receptor phr

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

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Gene Symbol

NR5A2 / LRH-1

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Protocol Information

IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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