NSC95397

SKU: orb1300095

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Description

NSC 95397 is a potent small-molecule inhibitor of Cdc25 dual-specificity phosphatases, with Ki values in the nanomolar range for Cdc25A, B, and C. It is widely used in biochemical and cell-based research to study cell cycle regulation, proliferation, and as a potential anticancer agent in experimental models.

Research Area

Cell Biology

Images & Validation

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Key Properties

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CAS Number	93718-83-3
MW	310.39
Purity	>98%
Formula	C₁₄H₁₄O₄S₂
SMILES	OCCSC1=C(SCCO)C(=O)c2ccccc2C1=O
Target	Phosphatase
Solubility	Soluble in DMSO (up to 15 mg/ml) or in Ethanol (up to 1 mg/ml).

Bioactivity

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Target IC50	CDC25A:32nM(ki)\|CDC25B:96nM(ki)\|CDC25C:40 nM(ki)
In Vitro	NSC 95397 reduced cell viability and anchorage-independent growth of all the three colon cancer cell lines through inhibited proliferation and induced apoptosis via regulating cell-cycle-related proteins, including p21, cyclin-dependent kinases, and caspases. Read more...
Cell Research	To measure the cell proliferation activity of NSC 95397 against colon cancer cells, SW480 (1 × 10^4), SW620 (2 × 10^4), and DLD-1 (1 × 10^4) cells were seeded into 96-well plates. After overnight culture, NSC 95397 cells were added at the indicated concentrations. After 24 h of incubation, cell proliferation was determined in vitro using a BrdU cell proliferation assay kit. O.D. 450 values were analyzed by using a Multiskan PC.The apoptosis of colon cancer cells was determined by a PE Annexin V Apoptosis Detection Kit with 7-AAD. Briefly, cells were treated with the indicated concentration of NSC 95397 for 24 h. After 24 h, Cells were harvested and stained with PE Annexin V/7-AAD for 15 min. The stained cells were analyzed using FACSCanto II low cytometer and FCS Express software Read more...

Storage & Handling

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Storage	-20°C
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Phosphatase, Apoptosis, NSC 95397, inhibit, NSC95397, NSC-95397, Inhibitor

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Compound Identifiers

PubChem CID

262093 ↗

Key Properties

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No computed properties available.

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NSC95397 (orb1300095)

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NSC95397

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

Quality Guarantee

Compound Identifiers

Key Properties

Documents Download

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Protocol Information

NSC95397 (orb1300095)

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