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Description
Research Area
Metabolism Research
Images & Validation
−Item 1 of 2
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2b |
| Clone No. | NSEL1-1 |
| Immunogen | Amino acids 416-433 of human Neuron-specific Enolase were used as the immunogen for this NSE antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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IHC testing of FFPE human pancreas with NSE antibody (clone NSEL1-1). Staining of FFPE tissue requires boiling sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human HeLa cell lysate with NSE antibody (clone NSEL1-1). Predicted molecular weight ~47 kDa.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
NSE Antibody (orb2641986)
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