Nucleolin Antibody

SKU: orb2638988

Description

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.

Images & Validation

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Tested Applications	FACS, IF, IHC-P, WB
Dilution range	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human, Mouse, Rat
Application Notes	Optimal dilution of the Nucleolin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	NCL/902
Immunogen	Recombinant human protein was used as the immunogen for the Nucleolin antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Western blot of A431 cell lysate using Nucleolin antibody.

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with Nucleolin antibody.

IHC: Formalin-fixed, paraffin-embedded human skin stained with Nucleolin antibody.

IHC: Formalin-fixed, paraffin-embedded human uterus stained with Nucleolin antibody.

IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Nucleolin antibody.

IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Nucleolin antibody.

Flow cytometry of human Jurkat cells. Grey: isotype control; Green: FITC-labeled; Purple: APC-Labeled; turquoise: AF700-Labeled Nucleolin antibody.

Flow cytometry testing of human 293T cells. Black: cells alone; Grey: isotype control; Green: AF488-labeled Nucleolin antibody.

Permeabilized human HeLa cells stained with AF488 labeled Nucleolin antibody. Green: AF488-labeled Ab. Blue: DAPI.

Immunofluorescent staining of PFA-fixed human MCF7 cells with Nucleolin antibody (green, clone NCL/902) and Phalloidin (red).

Quick Database Links

UniProt

P19338

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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