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Description
Research Area
Epigenetics & Chromatin
Images & Validation
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Item 1 of 2
| Tested Applications | ELISA, IHC, IP, WB |
|---|---|
| Dilution Range | ELISA: 1:5,000 - 1:20,000, IHC: 1:200 - 1:1,000, IP: 1:100, WB: 1:500 - 1:2,000 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Antiserum |
| Immunogen | Rb2 (p130) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Target | RBL2 |
| Purity | This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 130 kDa Rb2 protein from human, rat and mouse tissue. No reaction was observed against other related tumor suppressor proteins. Cross reactivity with Rb2 (p130) from other species may also occur. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 85 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-p130 Antibody, rabbit anti-Rb2 antibody, RBR 2 antibody, RBR2 antibody, Retinoblastoma like 2 antibody, Retinoblastoma like protein 2 antibody, Retinoblastoma Related Gene antibody
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RBL2 Rabbit Polyclonal Antibody [orb576773]
WB
Bovine, Canine, Equine, Guinea pig, Rabbit, Rat, Zebrafish
Human, Mouse
Rabbit
Polyclonal
Unconjugated
100 μl - Item 1 of 1
Quality Guarantee
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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Usually dispatched within 5-10 working days