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p38 (phospho Thr180) rabbit pAb Antibody
SKU: orb764254
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Description
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−Item 1 of 4
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | WB 1:500-2000, IF 1:50-300, IHC 1:50-300 IHC 1:50-300 |
| Reactivity | Human, Monkey, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p38 MAPK around the phosphorylation site of Thr180. AA range:147-196 |
| Molecular Weight | 38 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-MAPK14 antibody, anti-CSBP antibody, anti-CSBP1 antibody, anti-CSBP2 antibody, anti-CSPB1 antibody, anti-MXI2 antibody, anti-SAPK2A antibody, anti-Mitogen-activated protein kinase 14 antibody, anti-MAP kinase 14 antibody, anti-MAPK 14 antibody, anti-Cytokine suppressive anti-inflammatory drug-binding protein antibody, anti-CSAID-binding protein antibody, anti-CSBP antibody, anti- antibody
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Immunofluorescence analysis of Hela cell. 1, p38 (phospho Thr180) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.
Immunofluorescence analysis of rat-lung tissue. 1, p38 (phospho Thr180) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.
Immunofluorescence analysis of rat-lung tissue. 1, p38 (phospho Thr180) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, p38 (phospho Thr180) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
p38 (phospho Thr180) rabbit pAb Antibody (orb764254)
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