p53 Antibody (Biotin)

SKU: orb377405

Description

Rabbit polyclonal antibody against p53 conjugated to Biotin.

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Molecular Biology, Signal Transduction

Images & Validation

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Tested Applications	ICC, IF, WB
Dilution range	WB (1:1000); ICC/IF (1:100)
Reactivity	Human
Application Notes	A 1:1000 dilution of SPC-682 was sufficient for detection of p53 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide from the C-terminal of human tumor protein p53.
Target	p53
Molecular Weight	53kDa
Purification	Peptide Affinity Purified
Conjugation	Biotin

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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TP53, Tumor Protein 53, BCC7, LFS1, TRP53, p53 tumor suppressor

Quality Guarantee

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Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p53 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-p53 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm, PML body, Endoplasmic Reticulum. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) p53 Antibody (D) Composite.

Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~43.7kDa p53 protein using Rabbit Anti-p53 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 μg). Lane 3: Human 293T (20 μg). Load: 20 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-p53 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~43.7kDa.

Western blot analysis of Human A431 showing detection of ~43.7kDa p53 protein using Rabbit Anti-p53 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human A431 (20 μg). Load: 20 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-p53 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~43.7kDa.

Quick Database Links

Gene Symbol

p53

UniProt

P04637

NCBI Gene ID

7157

RefSeq (Protein):NP_000537.3

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000537.3

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ICC

Immunocytochemistry

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