PABPC4L Human Over-expression Lysate

SKU: orb1361583

Description

PABPC4L HEK293T cell transient overexpression lysate (as WB positive control)

Images & Validation

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Item 1 of 1

Application Notes

HEK293T cells in 10-cm dishes were transiently transfected withMegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. To facilitate transportation and protein, the products are supplied as lyophilized proteins.

Key Properties

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Source	Human
Expression System	HEK293T
Immunogen	Full-length peptide sequence
Target	PABPC4L
Tag	C-Myc/DDK
Molecular Weight	47.9 kDa

Storage & Handling

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Storage	The lysate can be shipped at ambient temperature. Upon receiving, store the sample at - 20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freezethaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C
Form/Appearance	1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
Disclaimer	For research use only

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Western blot validation of Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with transfection reagent MegaTran 2.0) using PABPC4L Human Over-expression Lysate

Quick Database Links

Gene Symbol

PABPC4L

RefSeq (mRNA):NM_001114734

RefSeq (Protein):NP_001108206

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

mRNA	NM_001114734
Protein	NP_001108206

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