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Description
Images & Validation
−Item 1 of 4
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1,κ |
| Immunogen | This PAH antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 127-161 amino acids from the Central region of human PAH. Antigen Region: 127-161 aa. |
| Target | PAH |
| Molecular Weight | 51862 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Phenylalanine-4-hydroxylase, PAH, Phe-4-monooxygenase, PAH
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Immunohistochemical analysis of paraffin-embedded H. liver section using PAH Antibody (Center). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded M. kidney section using PAH Antibody (Center). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. kidney section using PAH Antibody (Center). Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from mouse liver, rat liver tissue, HepG2 cell line (from left to right), using PAH Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Quick Database Links
Gene Symbol
PAH
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
PAH Antibody (Center) (orb1788416)
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