Pan-Cytokeratin, 2 Clones (AE1/AE3) Antibody

SKU: orb27182

Description

Mouse monoclonal antibody to pan Cytokeratin

Images & Validation

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Tested Applications	IHC-Fr, IHC-P
Dilution Range	(liquid conc.) 1:20 - 1:50
Reactivity	Human
Application Notes	Positive Control: Appendix. Pre-Treatment: Paraffin sections may be pre-treated with 1 % protease solution (Unmasking fluid P, Art.-No. DE110) for 15 min at RT, improvement of staining may be achieved with Unmasking Fluid G (Art. No. DE007) or Unmasking Fluid C (Art. No. DE000). Incubation Time: 60 min at RT

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	AE1/AE3
Immunogen	Human epithelial keratins
Purification	Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 200 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Concentration	70 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Pan-Cytokeratin, 2 Clones (AE1/AE3) Antibody

Immunohistochemical staining of human epidermis using pan Cytokeratin antibody

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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