Pan SUMO Antibody

SKU: orb30940

This product can be custom made

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Description

Purified rabbit polyclonal antibody.

Research Area

Cell Biology

Images & Validation

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Item 1 of 4

Tested Applications	IF, IHC-P, WB
Dilution Range	IF - 1:10-50, WB - 1:1000, IHC-P - 1:100
Reactivity	Human, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	Recombinant Protein
Target	SUMO3
Molecular Weight	11637
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti-Small ubiquitin-related modifier 1 antibody, anti-SUMO-1 antibody, anti-Ubiquitin-like protein SMT3C antibody, anti-SMT3 homolog 3 antibody, anti-Ubiquitin-homology domain protein PIC1 antibody, anti-Ubiquitin-like protein UBL1 antibody, anti-GAP modifying protein 1 antibody, anti-GMP1 antibody, anti-Sentrin antibody, anti-SMT3C antibody, anti-SMT3H3 antibody, anti-UBL1 antibody, anti-Small ubiquitin-related modifier 2 antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Fluorescent confocal image of Hela cell stained with Pan SUMO Antibody. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Pan SUMO primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). Pan SUMO immunoreactivity is localized to Nucleus significantly.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunohistochemical analysis of paraffin-embedded H.bladder section using Pan SUMO Antibody. diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

SUMO3 Antibody western blot analysis in 293 cell line and rat liver tissue lysates (35 ug/lane). This demonstrates the SUMO3 antibody detected the SUMO3 protein (arrow).