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Papain Antibody
SKU: orb344340
Description
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| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:3,000, WB: 1:1,000 - 1:5,000 |
| Reactivity | Plant |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-Papain Antibody was produced by repeated immunizations with pork Papain protein isolated from papaya. |
| Purity | Anti-Papain antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Papain [Carica papaya]. Cross reactivity against Papain from other sources may occur but has not been specifically determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−goat anti-Papain Antibody, Allergen Car p 1 antibody, Papaya proteinase I antibody, PPI antibody
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Western Blot of Goat anti-Papain Antibody. Lane 1: Papain. Load: 50 ng per lane. Primary antibody: Goat anti-Papain Antibody at 1:1000 overnight at 4°C. Secondary antibody: HRP goat secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 23 kDa, 23 kDa for Papain.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Papain Antibody (orb344340)
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