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Papain Antibody Peroxidase Conjugated

SKU: orb344363

Description

Papain antibody (Peroxidase)

Images & Validation

Tested ApplicationsDOT, ELISA, IHC, WB
Dilution rangeELISA: 1:1,000 - 1:5,000, IHC: 1:200- 1:1,000, WB: 1:500- 1:2,500
ReactivityPlant
Application Notes
Anti-Papain peroxidase antibody has been tested by ELISA, dot blot, and western blot. This product is assayed against 1.0 ug of Papain [Carica papaya] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
IsotypeIgG
ImmunogenAnti-Papain Antibody was produced by repeated immunizations with pork Papain protein isolated from papaya.
PurityAnti-Papain is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Papain [Carica papaya]. Cross reactivity against Papain from other sources may occur but has not been specifically determined.
ConjugationHRP

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/Preservatives0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Concentration1.0 mg/mL
DisclaimerFor research use only

Alternative Names

goat anti-Papain Antibody HRP Conjugated, Peroxidase Conjugated goat anti-Papain Antibody, Allergen Car p 1 antibody, Papaya proteinase I antibody, PPI antibody

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Papain Antibody Peroxidase Conjugated

Western Blot of Goat anti-Papain Antibody Peroxidase Conjugated. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: Papain. Load: 50 ng per lane. Primary antibody: Goat anti-Papain Antibody Peroxidase Conjugated at 1:1000 overnight at 4°C. Secondary antibody: none. Block: orb348637 for 30 min at RT. Predicted/Observed size: 23.4 kDa, >25 kDa for Papain.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP00784.1

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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DOT
Dot Blot
View Protocol

Papain Antibody Peroxidase Conjugated (orb344363)

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100 μg
¥ 11,180.00
DispatchUsually dispatched within 5-10 working days
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