Queen's Awards Received in 2021 ISO 9001 Certified Delivered over 1,000,000 bio-reagents to life science researchers Trusted by Life Science Communities
Cart summary

You have no items in your shopping cart.

Pemetrexed

SKU: orb1310854

Description

Pemetrexed

Research Area

Pharmacology & Drug Discovery

Images & Validation

−
Pemetrexed

Key Properties

−
CAS Number137281-23-3
MW427.41
Purity>99.99% (May vary between batches)
FormulaC20H21N5O6
SMILESNc1nc(=O)c2c(CCc3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O)c[nH]c2[nH]1
TargetAntifolate,Autophagy,DHFR,DNA/RNA Synthesis
Solubility10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.68 mM);DMSO:35 mg/mL (81.89 mM)

Bioactivity

−
Target IC50
TS:1.3 nM(ki)|DHFR:7.2 nM(ki)|GARFT:65 nM(ki)
In Vivo
The group of mice treated with PC61 combined with Pemetrexed exhibited significantly longer survival compared to those treated with PC61 alone, rat IgG combined with Pemetrexed, or left untreated. Survival analysis further confirmed that the PC61 and Pemetrexed combination resulted in significantly improved survival rates .
Read more...
In Vitro
Pemetrexed (LY231514) disodium is a novel classical antifolate whose antitumor activity likely results from the simultaneous inhibition of several key folate-requiring enzymes via its polyglutamated metabolites. It is one of the best substrates for the enzyme FPGS (Km=1.6 μM, Vmax/Km=621). Polyglutamation and the polyglutamated metabolites of LY231514 play significant roles in determining its selectivity and antitumor activity. LY231514 only moderately inhibits TS (Ki=340 nM, recombinant mouse), but its pentaglutamate form is 100-fold more potent (Ki=3.4 nM), making it one of the most potent folate-based TS inhibitors.
Read more...
Cell Research
Pemetrexed is dissolved in DMSO and stored, and then diluted with cell culture medium before use. Dose-response curves are generated to determine the concentration required for 50% inhibition of growth (IC50). Pemetrexed is dissolved initially in DMSO at a concentration of 4 mg/mL and further diluted with cell culture medium to the desired concentration. CCRF-CEM leukemia cells in complete medium are added to 24-well Cluster plates at a final concentration of 4.8×l04 cells/well in a total volume of 2 mL. Test compounds at various concentrations are added to duplicate wells so that the final volume of DMSO is 0.5%. The plates are incubated for 72 h at 37°C in an atmosphere of 5% CO2 in air. At the end of the incubation, cell numbers are determined on a ZBI Coulter counter. Control wells usually contain 4×105 to 6×105 cells at the end of the incubation. For several studies, IC50s are determined for each compound in the presence of either 300 μM AICA, 5 μM thymidine, 100 μM hypoxanthine, or combination of 5 μM hymidine plus 100 μM hypoxanthine.
Read more...

Storage & Handling

−
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

−
LY-231514 Disodium Hydrate, LY231514, LY231514 Disodium Hydrate, GARFT, Autophagy, Antifolate, DNASynthesis, DHFR, DNA Synthesis, Thymidylate synthase, Pemetrexed acid, RNASynthesis, RNA Synthesis

Similar Products

−
    1. Pemetrexed Disodium Hemipentahydrate
    Item 1 of 1

    Pemetrexed Disodium Hemipentahydrate [orb134833]

    >99%

    357166-30-4

    1032.84

    2(C20H19N5NA2O6).5(H2O)

    250 mg, 100 mg, 1 g
  • LY-338979

    LY-338979 [orb1705685]

    193281-00-4

    443.41

    C20H21N5O7

    25 mg, 100 mg, 50 mg
  • Pemetrexed disodium heptahydrate

    Pemetrexed disodium heptahydrate [orb3027182]

    99.78% (May vary between batches)

    357166-29-1

    597.49

    C20H33N5Na2O13

    500 mg, 50 mg, 100 mg, 25 mg
  • Pemetrexed 2Na

    Pemetrexed 2Na [orb340485]

    >98%

    357166-29-1

    597.5

    C20H19N5Na2O6 [7H2O]

    10 mg, 50 mg
  • Pemetrexed disodium

    Pemetrexed disodium [orb1310853]

    99.92% (May vary between batches)

    150399-23-8

    471.37

    C20H19N5Na2O6

    50 mg, 100 mg, 200 mg, 1 ml x 10 mM (in DMSO), 25 mg, 500 mg, 1 g
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Compound Identifiers

PubChem CID
135410875 ↗

Key Properties

−
No computed properties available.

Documents Download

Datasheet
Product Information
Download

Request a Document

Protocol Information

Find More Protocols

Pemetrexed (orb1310854)

  • Star
  • Star
  • Star
  • Star
  • Star
  • 0.0
Based on 0 reviews

Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.

Login to Submit a Review

No reviews yet

Login to review

Step 1: Enter information below

(Recommended: An additional animal making an allowance for loss during the experiment)

Step 2: Enter the in vivo formulation

(This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO +
%+
% Tween 80 +
%

Available Sizes

Select a size below

25 mg
¥ 1,040.00
50 mg
¥ 1,170.00
1 ml x 10 mM (in DMSO)
¥ 1,300.00
100 mg
¥ 1,430.00
200 mg
¥ 1,820.00
500 mg
¥ 2,470.00
DispatchUsually dispatched within 3-5 working days
Bulk Enquiry
Need technical support? Find your Distributor Return and Tax Policy

Email

[email protected]

UK Office

+44 (0)1223 859353

US Office

+1 (415) 906-5211

CN Office

+86 (27)-87663101

Distributors

biorbyt.com/distributor