PGP9.5 / UCHL1 antibody

SKU: orb2276379

Description

PGP9.5 / UCHL1 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

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Item 1 of 3

Tested Applications	IHC
Dilution range	1:100-1:200
Reactivity	Human
Application Notes	Positive Control: Pancreas: Islets cells and interspersed nerve fibres should show strong PGP9.5 staining. Negative Control: Pancreas: Acinar cells and excretory ducts should not show any PGP9.5 immunostaining. Cellular Localization: Cytoplasmic. Endoplasmic Reticulum membrane Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions. Read more...

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone No.	MSVA-905R
Immunogen	Recombinant full-length human UCHL1 protein
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

Alternative Names

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Gracile Axonal Dystrophy; Neuron Cytoplasmic Protein 9.5; Park5; Parkinson Disease 5; PGP95; Protein Gene Product 9.5; Ubiquitin Carboxyl-terminal Esterase L1; Ubiquitin Carboxyl- terminal Hydrolase Isozyme L1; Ubiquitin Thioesterase L1; Ubiquitin Thiolesterase L2

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Colon descendes muscular wall Strong PGP9.5 immunostaining of nerve fibres and ganglions in the muscular wall of the colon descencens.

Medullary thyroid cancer showing strong PGP9.5 immunostaining.

Testis - In the testis, a strong PGP9.5 immunostaining is seen in spermatogonia. PGP9.5 staining decreases sharply in spermatocytes where it is only faint. A moderate PGP9.5 staining is seen in Leydig cells while Sertoli cells remain PGP9.5 negative.

Quick Database Links

UniProt

P09936

UniProt Details

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No UniProt data available

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Protocol Information

IHC

Immunohistochemistry

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