Recombinant UchL1 Antibody

SKU: orb639550

Description

This mAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.

Research Area

Epigenetics, Neuroscience

Images & Validation

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Tested Applications	FACS, IF, IHC-P, WB
Dilution range	Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1)
Reactivity	Human
Application Notes	Optimal dilution of the recombinant UchL1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	rUBCE-L1
Immunogen	Recombinant human protein was used as the immunogen for the recombinant UchL1 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC staining of FFPE human cerebellum with recombinant UchL1 antibody (clone rUBCE-L1). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of permeabilized human T98G cells with recombinant UchL1 antibody cocktail (green, clone rUBCE-L1) and Nucspot (red).

Western blot testing of human brain lysate with recombinant UchL1 antibody (clone rUBCE-L1). Predicted molecular weight ~25 kDa.

Flow cytometry testing of permeabilized human T98G cells with recombinant UchL1 antibody (clone rUBCE-L1); Red=isotype control, Blue= recombinant PGP9.5 antibody.

Quick Database Links

UniProt

P09936

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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