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PHGDH Recombinant Mouse Monoclonal Antibody
SKU: orb1172535
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | IgG1, Kappa |
| Clone No. | D12E1 |
| Target | PHGDH |
| Molecular Weight | 56 kDa |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−3-phosphoglycerate dehydrogenase; D-3-phosphoglycerate dehydrogenase; EC 1.1.1.95; PDG; PGAD; PGD; PGDH; PGDH3; SERA; NLS; NLS1; 3PGDH; 3-PGDH; 3 PGDH; PHGDHD; HEL-S-113; SERA_HUMAN; EC:1.1.1.95; EC:1.1.1.399; EC:1.1.1.37; Malate dehydrogenase; 2-oxoglutarate reductase.
Quality Guarantee
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: room temperature 2h, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: HeLa, BRL, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 57 kDa, Observed MW: 57 kDa.
Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172535.
Quick Database Links
Gene Symbol
PHGDH
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
PHGDH Recombinant Mouse Monoclonal Antibody (orb1172535)
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