Phospho-AMPK alpha 1 (Ser496) Recombinant Rabbit Monoclonal Antibody

SKU: orb559194

Description

Images & Validation

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Item 1 of 1

Tested Applications	ICC, WB
Dilution range	WB=1:500-2000, ICC/IF=1:50-200
Reactivity	Human
Predicted Reactivity	Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	11G1
Immunogen	KLH conjugated Synthesised phosphopeptide derived from mouse AMPK alpha 1 around the phosphorylation site of Ser496 SG(p-S)VS
Target	PRKAA1
Molecular Weight	64 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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p-AMPK alpha 1(S496); phospho-AMPK alpha-1(S496); AMPK alpha-1(phospho-S496); AMPK alpha 1(phospho S496); AMPK alpha-1; 5 AMP activated protein kinase alpha 1catalytic subunit; 5 AMP activated protein kinase catalytic alpha 1 chain; 5' AMP activated protein kinase catalytic subunit alpha 1; AAPK1; acetyl CoA carboxylase kinase; AI194361; AI450832; AL024255; AMP-activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK 1; AMPK alpha 1 chain; AMPK; AMPK1; AMPKa1; AMPKalpha1; C130083N04Rik; cb116; EC 2.7.11.1; HMG CoA reductase kinase; hormone sensitive lipase kinase; im:7154392; kinase AMPK alpha1; MGC33776; MGC57364; PRKAA 1; PRKAA1; Protein kinase AMP activated alpha 1 catalytic subunit; SNF1-like protein AMPK; wu:fa94c10; AAPK1_HUMAN; AMPKα1; AMPK α1; AMPKα 1; AMPK α 1; AMPK AMPK-α1; AMPK-α-1; α-1; AMPK-a; AMPK a1.

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Phospho-AMPK alpha 1 (Ser496) Recombinant Rabbit Monoclonal Antibody

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: Huvec (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-AMPK alpha 1 (Ser496) (orb559194) at 1/1000 dilution, Anti-beta-Actin (orb500817) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63 kD, Observed band size: 60 kD.

Quick Database Links

Gene Symbol

PRKAA1

UniProt

Q5EG47

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ICC

Immunocytochemistry

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