Phospho-c-Jun (Ser63) Recombinant Rabbit Monoclonal Antibody

SKU: orb559120

This product can be custom made

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Description

Phospho-c-Jun (Ser63) Recombinant Rabbit Monoclonal Antibody

Research Area

Cancer Biology

Images & Validation

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Item 1 of 4

Tested Applications	ICC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:50-500, IHC-F=1:50-500, ICC/IF=1:50-200, IF=1:50-500
Reactivity	Human, Mouse
Predicted Reactivity	Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	6C2
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human c-Jun around the phosphorylation site of Ser63 LT(p-S)PD
Target	JUN
Molecular Weight	43/36 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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JUN | c-Jun (p-S63); p-c-Jun; phospho-c-Jun; AP-1; AP1; c-Jun; cJUN; p39; Junc; JUN_HUMAN; JUN; Activator protein 1 (AP1); Proto-oncogene c-Jun; Transcription factor AP-1 subunit Jun; V-jun avian sarcoma virus 17 oncogene homolog; JUN_MOUSE; AH119; V-jun avian sarcoma virus 17 oncogene homolog (Jun A); JUN_PIG; JUN_RAT; Rjg-9;

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Phospho-c-Jun (Ser63) Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-c-Jun (Ser63)) Monoclonal Antibody, Unconjugated (orb559120) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-c-Jun (Ser63)) Monoclonal Antibody, Unconjugated (orb559120) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human skin cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-c-Jun (Ser63)) Monoclonal Antibody, Unconjugated (orb559120) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 2: Hela (Human) Cell Lysate at 30 ug, Lane 3: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 4: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-c-Jun (Ser63) (orb559120) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36/43 kD, Observed band size: 43 kD.