Phospho-CREB-1 (Ser121) Rabbit Polyclonal Antibody

SKU: orb4982

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Description

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Tested Applications	FC, WB
Dilution range	WB=1:500-2000, Flow-Cyt=1μg/Test
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Equine, Gallus, Porcine, Rat, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human CREB-1 around the phosphorylation site of Ser121 TD(p-S)QK
Target	CREB1
Molecular Weight	37 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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CREB(phospho-Ser121); p-CREB-1(Ser121); pCREB-1(Ser121); Active transcription factor CREB; cAMP response element binding protein; cAMP responsive element binding protein 1; CREB1; CREB 1; CREB1; MGC9284; Transactivator protein; CREB; CREB1_HUMAN.

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Phospho-CREB-1 (Ser121) Rabbit Polyclonal Antibody

Blank control (blue line): Hela cells (fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C). Primary Antibody (green line): Rabbit Anti-phospho-CREB-1 (Ser121) antibody (orb4982), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Cerebellum (Mouse) Lysate at 40 ug Lane 3: Kidney (Mouse) Lysate at 40 ug Primary: Anti-phospho-CREB-1 (Ser121) (orb4982) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD.

Quick Database Links

Gene Symbol

CREB1

UniProt

P16220

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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