Phospho-CrkL (Tyr207) (G4) rabbit mAb Antibody

SKU: orb1946278

Description

CrkL (v-Crk sarcoma virus CT10 oncogene-like protein) is an adaptor protein composed of one Src Homology 2 (SH2) and two Src Homology 3 (SH3) domains separated by flexible linker sequences that act as building blocks to assemble multiprotein complexes (1). The Crk adaptor proteins (Crk and CrkL) constitute an integral part of a network of essential signal transduction pathways in humans and other organisms that act as major convergence points in tyrosine kinase signaling. CRKL is required for the normal development of multiple tissues that rely on fibroblast growth factor 8 (FGF8). Phosphorylation of Crk on Tyr 221 or CrkL on Tyr 207 causes intramolecular binding of the linker region to the SH2 domain, sequestering the SH2 and SH3N and preventing them from binding target proteins (2,3). Mounting evidence indicates that dysregulation of Crk proteins is associated with human diseases, including cancer and susceptibility to pathogen infections.

Images & Validation

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Item 1 of 6

Tested Applications	FC, WB
Dilution range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human, Mouse

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	CrkLY207-G4
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Tyr207 of human phospho CrkL
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/mL
Disclaimer	For research use only

Alternative Names

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Crk-like protein; CRKL; v-crk sarcoma virus CT10 oncogene homolog (avian)-like

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Phospho-CrkL (Tyr207) (G4) rabbit mAb Antibody

Flow cytometric analysis of K562 cells treated with imatinib (red) or treated with pervanadate (green) using Phospho-CRKL (Tyr207) (G4) Rabbit mAb CRKLY207-G4 #orb1946278 at 0.05 ug/mL, or concentration-matched Rabbit (G9) mAb IgG Isotype Control #orb1946241 for cells treated with imatinib (black) or treated with pervanadate (blue).

Western blot analysis of K562 cell extract untreated or treated with imatinib using 1ng/mL CrkL (Tyr207) antibody CrkLY207-G4. Cat. #orb1946278.

Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or treated with imatinib (red) or treated with pervanadate (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-CrkL (Tyr207) antibody CrkLY207-G4 at 0.01μg/mL. Cat. #orb1946278.

Western blot analysis of NIH3T3 cell extract using 1μg/mL Phospho-CrkL (Tyr207) antibody CrkLY207-G4. Cat. #orb1946278.

Western blot of E10.5 mouse wild-type (+/+) or Crkl knock out (-/-) whole embryos. The red channel was stained using a β-actin loading control and the green channel was stained using 1:500 dilution of Phospho-CrkL (Tyr207) antibody CrkLY207-G4 Cat. #orb1946278. Phospho CrkL antibody staining is absent in the knock out lysate.

Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using 0.05 μg/mL of Phospho-CrkL (Tyr207) antibody CrkLY207-G4 (Cat. #orb1946278) or Company C antibody at 0.1ug/mL.

Quick Database Links

UniProt

P46109

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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