Phospho-EGFR (Tyr1068) rabbit mAb Antibody

SKU: orb1946283

Description

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is a transmembrane protein that is a receptor for members of the epidermal growth factor family (EGF family) of extracellular protein ligands (1). EGFR (rbB-1) is closely related to other members of the ErbB family of receptors: HER2/neu(ErbB-2), HER3 (ErbB-3) and HER4 (ErbB-4). In many cancer types, mutations affecting EGFR expression or activity could result in cancer (2). Overexpression of EGFR is associated with the development of a wide variety of tumors. Interruption of EGFR signaling, either by blocking EGFR binding sites on the extracellular domain of the receptor or by inhibiting intracellular tyrosine kinase activity, can prevent the growth of EGFR-expressing tumors and improve the patient's condition. EGFR is activated by the binding of its ligands including EGF and dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity. Activation of EGFR leads to autophosphorylation of tyrosine (Tyr) residues; Tyr992, Tyr1045, Y1068, Tyr1148, and Tyr1173 in the C-terminal domain.

Research Area

Cell Biology

Images & Validation

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Item 1 of 6

Tested Applications	FC
Dilution Range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	BG7
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human phospho EGFR.
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EGFR; Epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); ERBB; ERBB1; HER1; mENA; PIG61; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

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Phospho-EGFR (Tyr1068) rabbit mAb Antibody

Flow cytometric analysis of C6 cells secondary antibody only (blue) treated with imatinib (grey) with 0.1 μg/mL of isotype control Cat. #orb1946241 or imatinib (red) or pervanadate (green) using 0.1 μg/mL of Phospho-EGFR (Tyr1068) antibody EGFRY1068-E5 (green) Cat. #orb1946283.

Flow cytometric analysis of HeLa cells secondary antibody only (blue) treated with imatinib (grey) or pervanadate (orange) with 0.1 μg/mL of isotype control Cat. #orb1946241 or imatinib (red) or pervanadate (green) using 0.1 μg/mL of Phospho-EGFR (Tyr1068) antibody EGFRY1068-E5 (green) Cat. #orb1946283.

Flow cytometric analysis of 3T3 cells secondary antibody only (blue) treated with imatinib (grey) or pervanadate (orange) with 0.1 μg/mL of isotype control Cat. #orb1946241 or imatinib (red) or pervanadate (green) using 0.1 μg/mL of Phospho-EGFR (Tyr1068) antibody EGFRY1068-E5 (green) Cat. #orb1946283.

Peptide blocking flow cytometric analysis of HeLa cells secondary antibody only negative control (light blue) or treated with imatinib (red) or with pervanadate (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-EGFR (Tyr1068) antibody EGFRY1068-E5 at 0.01μg/mL. Cat. #orb1946283.

Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or untreated (red) or treated with EGF and pervanadate (green) using Phospho-EGFR (Tyr1068) antibody at 0.01 μg/mL EGFRY1068-E5. Cat. #orb1946283.

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UniProt

P00533

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No UniProt data available

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Protocol Information

Flow Cytometry

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