Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human EFHC1 around the phosphorylation site of Ser539 PD(p-S)R
Target	EIF2B5
Molecular Weight	80 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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EIF2B5 | eIF2B epsilon (p-S539); p-eIF2B epsilon; phospho-eIF2B epsilon; CACH; CLE; EIF-2B; EIF2Bepsilon; LVWM; VWM5; EI2BE_HUMAN; EIF2B5; eIF2B GDP-GTP exchange factor subunit epsilon; EIF2BE; EI2BE_MOUSE; EI2BE_RAT;

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Phospho-eIF2B epsilon (Ser539) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p-eIF2B epsilon (Ser539)) Polyclonal Antibody, Unconjugated (orb183322) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human liver cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p-eIF2B epsilon (Ser539)) Polyclonal Antibody, Unconjugated (orb183322) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

EIF2B5

UniProt

Q13144

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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