Phospho Enol Pyruvate Carboxylase Antibody Peroxidase Conjugated

SKU: orb344895

Description

Enol Pyruvate Carboxylase antibody (Peroxidase)

Images & Validation

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Tested Applications	ELISA, WB
Dilution range	ELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000
Reactivity	Plant
Application Notes	Anti-Phosphoenolpyruvate Carboxylase Peroxidase conjugated antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Phospho-enol-Pyruvate Carboxylase [Maize] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for this product. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Phospho-enol-pyruvate Carboxylase [Maize Leaves]
Purity	Phospho Enol Pyruvate Carboxylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Phospho-enol-Pyruvate Carboxylase [Maize]. Cross reactivity against Phospho-enol-Pyruvate Carboxylase from other tissues and species may occur but have not been specifically determined.
Conjugation	HRP

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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rabbit anti-Phospho Enol Pyruvate Carboxylase Antibody Peroxidase Conjugation, PE conjugated rabbit anti-PEPC 1 antibody, PEPCase 1 antibody, Phosphoenolpyruvate carboxylase 1 antibody

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Quick Database Links

UniProt

B8XPZ2

RefSeq:ACN80021.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	ACN80021.1

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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