Phospho-ErbB2(Y1248) Antibody

SKU: orb1931272

This product can be custom made

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

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Item 1 of 3

Tested Applications	DOT, WB
Dilution Range	WB - 1:8000, DB - 1:500
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	This ErbB2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1248 of human ErbB2.
Target	ERBB2
Molecular Weight	137910 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, CD340, ERBB2, HER2, MLN19, NEU, NGL

Quality Guarantee

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Dot blot analysis of anti-Phospho-ErbB2-pY1248 (M) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Western blot analysis of extracts from A431 cells, untreated or treated with EGF, 100ng/ml, using phospho-ERBB2-Y1248 (left) or ERBB2 antibody (right)

Western blot analysis of extracts from A431 cells, untreated or treated with EGF, 100ng/ml, using phospho-ERBB2-pY1248 (L) (left) or ERBB2 antibody (right)

Quick Database Links

Gene Symbol

ERBB2

UniProt

P04626

RefSeq (Protein):NP_001005862.1, NP_004439.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001005862.1, NP_004439.2

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Protocol Information

Western Blot (IB, immunoblot)

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DOT

Dot Blot

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