Phospho-ETS1 (Thr38) Rabbit Polyclonal Antibody

SKU: orb156790

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Description

Research Area

Cardiovascular Research, DNA and RNA, DNA and RNA Research, Transcription Factors, Transcription Regulators

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse
Predicted Reactivity	Mouse

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthesised phosphopeptide derived from human ETS1 around the phosphorylation site of Thr38 LL(p-T)PS
Target	ETS1
Molecular Weight	49 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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ETS1(phospho T38); ETS1(phospho Thr38); p-ETS1(phospho T38); Avian erythroblastosis virus E26(v ets) oncogene homolog 1; C ets 1 protein; ETS 1; Ets protein; ETS1; ETS-1; ETS1 protein; ETS1_HUMAN; EWSR 2; EWSR2; FLJ10768; p54; Protein C-ets-1; v ets avian erythroblastosis virus E2 oncogene homolog; v ets avian erythroblastosis virus E26 oncogene homolog 1; v ets erythroblastosis virus E26 oncogene homolog 1; v-ets erythroblastosis virus E26 oncogene homolog 1.

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Phospho-ETS1 (Thr38) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ETS1 (Thr38)) Polyclonal Antibody, Unconjugated (orb156790) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: HepG2 (Human) Cell Lysate at 30 ug, Spleen (Mouse) Lysate at 40 ug, Lymph node (Mouse) Lysate at 40 ug, Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-ETS1 (Thr38) (orb156790) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kD, Observed band size: 50 kD.

Quick Database Links

Gene Symbol

ETS1

UniProt

P14921

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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