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Phospho-FLT3 (Tyr842) Rabbit Polyclonal Antibody

SKU: orb5253

Description

Phospho-FLT3 (Tyr842) Rabbit Polyclonal Antibody

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityRat
Predicted ReactivityHuman, Mouse, Rabbit

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human FLT3 around the phosphorylation site of Tyr842 SN(p-Y)VV
TargetFLT3
Molecular Weight113 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

Flt3/CD135(phospho Y842); p-Flt3(phospho Y842); p-CD135(phospho Y842); Flt3/CD135(phospho Y842); p-Flt3/CD135(phospho Y842); p-Flt3(phospho Y842); p-CD135(phospho Y842); Flt3/CD135(phospho Y842)CD135 antigen; Fetal liver kinase 2; FL cytokine receptor; Flk 2; Flk2; Flt 3; Flt3; FMS like tyrosine kinase 3; Fms related tyrosine kinase 3; Growth factor receptor tyrosine kinase type III; Stem cell tyrosine kinase 1; Stk 1; Stk1; Tyrosine protein kinase receptor FLT3; FLT3_HUMAN.

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Phospho-FLT3 (Tyr842) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (FLT3 (Tyr842)) Polyclonal Antibody, Unconjugated (orb5253) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

UniProt Details

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

Phospho-FLT3 (Tyr842) Rabbit Polyclonal Antibody (orb5253)

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50 μl
¥ 3,250.00
100 μl
¥ 5,070.00
200 μl
¥ 6,110.00
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