Phospho-IRAK1 (Thr209) Rabbit Polyclonal Antibody

SKU: orb157703

This product can be custom made

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Description

Phospho-IRAK1 (Thr209) Rabbit Polyclonal Antibody

Research Area

Immunology & Inflammation

Images & Validation

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Tested Applications	ELISA, IF, IHC-Fr, IHC-P
Dilution Range	IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
Reactivity	Human, Rabbit
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human IRAK1 around the phosphorylation site of Thr209 QG(p-T)CN
Target	Irak1
Molecular Weight	78 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IRAK1 (p-T209); p-IRAK1; phospho-IRAK1; IRAK; pelle; IRAK-1; IRAK1-S; IRAK1b; Il1rak; Plpk; mPLK; RGD1563841; IRAK1_HUMAN; IRAK1; 2.7.11.1; IRAK1_MOUSE; Pelle-like protein kinase (mPLK);

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Phospho-IRAK1 (Thr209) Rabbit Polyclonal Antibody

phosphopeptide, non phosphopeptide.

Tissue/Cell: Human myometrium tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-IRAK1 (Thr209) Polyclonal Antibody, Unconjugated (orb157703) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

Irak1

UniProt

P51617

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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