Phospho-Jak1 (Tyr1022/1023) (F11) rabbit mAb Antibody

SKU: orb1946035

Description

Jak1 plays an essential role in the IFN-Œ± and IFN-g response pathways and is tyrosine-phosphorylated upon cellular exposure to these signals. Jak1 oral inhibitors have been used to benefit patients with advanced myelofibrosis, where Jak1 was initially shown to be constitutively active in the peripheral blood cells of these patients. Targeted, small-molecule Jak inhibitors have also been used for treatment of rheumatoid arthritis. In cases of advanced melanoma, acquired resistance to PD-1 blockade drugs is associated with loss-of-functions of mutations in Jak1/2 genes. These mutations block interferon gamma signaling and prevent programmed death ligand 1 (PD-L1) expression in tumor cells.

Images & Validation

−

Item 1 of 2

Tested Applications	FC
Dilution Range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human, Mouse

Key Properties

−

Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	Jak1Y10221023-F11
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Tyr1022/1023 of human phospho-Jak1
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

Tyrosine-protein kinase JAK1, Janus kinase 1

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Phospho-Jak1 (Tyr1022/1023) (F11) rabbit mAb Antibody

Flow cytometric analysis of Jurkat cells untreated (red) or treated with IFNa + IL-4 + pervanadate (green) using Phospho-Jak1 (Tyr1034/1035) antibody Jak1Y10341035-F11 at 0.01μg/mL. Cat. #orb1946035, or concentration matched isotope control, Cat# orb1946241 for untreated (gray ) or treated with IFNa + IL4 + Pervanadate (blue).

Peptide blocking flow cytometric analysis of Jurkat cells secondary antibody only negative control (light blue) or untreated (red) or treated with IFNα + IL-4 + pervandadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-Jak1 (Tyr1022/1023) antibody Jak1Y10221023-F11 at 0.001μg/mL. Cat. #orb1946035.

Quick Database Links

UniProt

P23458

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Flow Cytometry

View Protocol

Find More Protocols