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Phospho-LC3C(S12) Antibody
SKU: orb1931205
Description
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−Item 1 of 5
| Tested Applications | DOT, WB |
|---|---|
| Dilution range | WB - 1:1000, DB - 1:500 |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 14272 Da |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
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Dot blot analysis of Phospho-LC3 (APG8a) - S12 Antibody and Nonphospho-LC3 (APG8a) Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Something like SH-SY5Y cells expressing GFP-LC3-WT or-S12D treated with rapamycin or vehicle for 1 h.
Immunoblots of SH-SY5Y cells treated with MPP+ for 24h. The data shows that treatment with MPP+ showed no significant change in level of LC3.
Immunoblots of SH-SY5Y cells treated with rapamycin for 1 h. The data shows that treatment with rapamycin showed no significant change in level of LC3.
Immunoblots of phosphorylated LC3 (phospho-LC3) in CHO cell culture. LC3 and LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with anti-phospho-LC3 S12 antibody. LC3 = microtubule-associated protein light chain-3; S12A = replacement of the amino acid position 12 serine of LC3 with alanine. WT = wildtype LC3-transfected cell lysates; S12A = LC3 S12A mutant-transfected cell lysates; Empty vector = vector with no LC3 gene. Molecular size: LC3-I = 16kDa, and LC3-II = 14 kDa
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Protocol Information
WB
Western Blot (IB, immunoblot)
DOT
Dot Blot
Phospho-LC3C(S12) Antibody (orb1931205)
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