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Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 3
| Tested Applications | DOT, WB |
|---|---|
| Dilution Range | WB: 1:1000, DOT: 1:500 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | Synthetic Peptide |
| Target | Erbb2 |
| Molecular Weight | 138579 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti Erbb2 antibody, anti Kiaa3023 antibody, anti Neu antibody, anti Receptor tyrosine-protein kinase erbB-2 antibody, anti Proto-oncogene Neu antibody, anti Proto-oncogene c-ErbB-2 antibody, anti p185erbB2 antibody, anti CD_antigen=CD340 antibody, anti Flags: Precursor antibody
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−- Item 1 of 1
Phospho-M ERBB2(Y1140) Antibody [orb1788332]
WB
Human, Mouse
Rabbit
Polyclonal
Unconjugated
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Immunofluorescent analysis of Kir6.2 (pT224) staining in HuvEc Cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Immunohistochemical analysis of Kir6.2 (pT224) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of Kir6.2 (pT224) expression in MCF7 (A), mouse liver (B), rat liver (C) whole cell lysates.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001003817.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
DOT
Dot Blot
Chen, Jing et al. A role for ErbB signaling in the induction of reactive astrogliosis Cell Discov, 3, 17044 (2017)
Phospho-M ERBB2(Y1140) Antibody (orb163604)
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