Phospho-MCAM (Tyr641) Rabbit Polyclonal Antibody

SKU: orb4800

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Description

Phospho-MCAM (Tyr641) Rabbit Polyclonal Antibody

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution Range	WB=1:500-2000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human MCAM around the phosphorylation site of Tyr641 EK(p-Y)ID
Target	MCAM
Molecular Weight	71 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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MCAM (p-Y641); p-MCAM; phospho-MCAM; CD146; HEMCAM; METCAM; MUC18; MelCAM; 1-gicerin; CD149; s-endo; s-gicerin; MUC18_HUMAN; MCAM; Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; MUC18_MOUSE; Gicerin; MUC18_RAT;

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Phospho-MCAM (Tyr641) Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Placenta tissue lysates, Lane 2: Mouse Kidney tissue lysates, Lane 3: Human Huvec cell lysates, Lane 4: Human Hela cell lysates, Lane 5: Human U2OS cell lysates, Lane 6: Human U87MG cell lysates, Primary: Anti-phospho-MCAM (Tyr641) (orb4800) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 71 kDa, Observed band size: 117 kDa.

Sample: Siha (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-MCAM (Tyr641) (orb4800) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 71 kD, Observed band size: 118 kD.

Quick Database Links

Gene Symbol

MCAM

UniProt

P43121

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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