Phospho NF kB p65 (S536) Rabbit Polyclonal Antibody

SKU: orb1292536

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Description

Rabbit Polyclonal antibody to NF-kB p65 Phospho-S536

Research Area

Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC, WB
Dilution Range	WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	A phospho specific peptide corresponding to residues surrounding S536 of human RelA
Target	Phospho-NF-kB p65 (S536)
Molecular Weight	65 kDa
Purity	≥95% as determined by SDS-PAGE
Purification	Immunogen affinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	liquid
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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NFKB3,p65,NF-kB p65,RELA,CMCU

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Phospho NF kB p65 (S536) Rabbit Polyclonal Antibody

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with orb1292536 (Phospho-NF-kB p65-S536 antibody) at dilution of 1:1000, treated by Calyculin A (100nM) at 37℃ for 30min after serum-starvation overnight.

Immunohistochemistry of paraffin-embedded rat brain tissue slide using orb1292536 at dilution of 1:100.

Quick Database Links

Gene Symbol

Phospho-NF-kB p65 (S536)

UniProt

Q04206

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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