Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) rabbit mAb Antibody

SKU: orb1946370

Description

Human Erk1 and Erk2 Ser/Thr kinases share 84% sequence identity and nearly all functions. These MAP kinases are activated in response to mitogens and growth factors as part of the Ras-Raf-MEK-ERK signal transduction cascade. This pathway regulates cell survival, differentiation, adhesion, cell cycle progression, and many other cellular processes. Upon phosphorylation, Erk1/2 translocate to the nucleus to activate transcription factors including c-Fos, Elk1, Ets1, and SP-1. There are more than 175 known cytoplasmic and nuclear substrates of Erk1/2. The Erk1/2 cascade is upregulated in many human cancers, even when oncogenic mutations are not found. Multiple small-molecule inhibitors of Erk1/2 have been developed, including ones targeting the ATP-binding site either competitively or irreversibly.

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

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Item 1 of 6

Tested Applications	FC, WB
Dilution Range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human, Mouse

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	BC33
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Thr202/Tyr204 of human phospho Erk1/2.
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Mitogen-activated protein kinase 3, MAPK3, ERK2, p44-MAPK, PRKM3, Mitogen-activated protein kinase 1, MAPK1, ERK1, p42-MAPK, PRKM1, PRKM2

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Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) rabbit mAb Antibody

Flow cytomeric analysis of jurket cells treated with U0126 (red) or TPA (green) and stained using Phospho-ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11. Cat. #orb1946370 or concentration match isotope control Cat#orb1946241 for cells treated with U0126 (gray) or treated with TPA (blue).

ERK12T202Y204-A11 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or of Phospho-ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11 (green) Cat. #orb1946370.

Western blot analysis of 293T cell extract treated with U0126 or TPA using Phospho-ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11 at 0.1 μg/mL. Cat. #orb1946370.

Western blot analysis of Ramos cell extract untreated or treated with U0126 followed by no treatment or treatment with PMA using Phospho-ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11. Cat. #orb1946370.

Flow cytometric analysis of Jurkat cells, secondary antibody only negative control (blue), or treated with U0126 (red) or with PMA (green) using Phospho-Erk1/2 (T202/Y204) antibody ERK12T202Y204-A11 (Cat. #orb1946370) or Company C antibody at 0.43 μg/mL (manufacturer's recommended concentration).

Western blot analysis of Ramos cells treated with U0126 or treated with TPA using 0.05 ug/mL of Phospho ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11 Cat. #orb1946370 or Company C antibody at recommended concentration of 0.34 ug/mL.

Quick Database Links

UniProt

P27361, P28482

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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