Phospho-PDCD4 (Ser457) Rabbit Polyclonal Antibody

SKU: orb312713

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Description

Research Area

Apoptosis, Cell Cycle

Images & Validation

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Item 1 of 2

Tested Applications	ICC, WB
Dilution range	WB=1:500-2000, ICC/IF=1:100-500
Reactivity	Human, Mouse
Predicted Reactivity	Equine, Gallus, Porcine, Rabbit, Rat, Sheep, Zebrafish

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthesised phosphopeptide derived from human PDCD4 around the phosphorylation site of Ser457 FV(p-S)EG
Target	PDCD4
Molecular Weight	52 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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PDCD4(phospho S457); p-PDCD4(phospho S457); Death up-regulated gene protein; Dug; H731; Ma3; MGC33046; MGC33047; Neoplastic transformation inhibitor; Neoplastic transformation inhibitor protein; Nuclear antigen H731; Nuclear antigen H731 like; Nuclear antigen H731 like protein; Nuclear antigen H731-like; PDCD 4; Pdcd4; PDCD4_HUMAN; Programmed cell death 4; programmed cell death 4(neoplastic transformation inhibitor); Programmed cell death protein 4; Protein 197/15a; Protein MA-3; Tis; Topoisomerase-inhibitor suppressed protein.

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Phospho-PDCD4 (Ser457) Rabbit Polyclonal Antibody

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-PDCD4 (Ser457)) polyclonal Antibody, Unconjugated (orb312713) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-phospho-PDCD4 (Ser457) (orb312713) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 52 kD.

Quick Database Links

Gene Symbol

PDCD4

UniProt

Q53EL6

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ICC

Immunocytochemistry

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