Phospho-PTPN11 (Tyr511) Rabbit Polyclonal Antibody

SKU: orb1227216

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Description

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Tested Applications	IF, IHC-Fr, IHC-P
Dilution range	IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500
Reactivity	Human
Predicted Reactivity	Bovine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep, Zebrafish

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human PTPN11 around the phosphorylation site of Tyr511 AQ(p-Y)RF
Target	PTPN11
Molecular Weight	68 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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PTN11_HUMAN; Tyrosine-protein phosphatase non-receptor type 11; PTP2C; SHPTP2; EC:3.1.3.48; Protein-tyrosine phosphatase 1D(PTP-1D); Protein-tyrosine phosphatase 2C(PTP-2C); SH-PTP2(SHP-2; Shp2); SH-PTP3; protein tyrosine phosphatase non-receptor type 11; CFC; NS1; JMML; BPTP3; PTP2C; METCDS;

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Phospho-PTPN11 (Tyr511) Rabbit Polyclonal Antibody

Measured by its binding ability in a indirect ELISA. Immobilized Human phospho-PTPN11 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human phospho-PTPN11 Antibody, the EC50 is 10715 ng/ml.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-PTPN11 (Tyr511)) Polyclonal Antibody, Unconjugated (orb1227216) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-PTPN11 (Tyr511)) Polyclonal Antibody, Unconjugated (orb1227216) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

PTPN11

UniProt

Q06124

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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