Phospho-SAPK/JNK (Thr183/Tyr185) rabbit mAb Antibody

SKU: orb1946073

Description

The SAPK/JNK pathway initiates apoptosis upon exposure to radiation, UV exposure, heat shock, oxidative stress, and other stressors. Upon exposure to environmental stress, the SAPK/JNK signaling pathway sequentially activates the proteins MEKK1, SEK1, SAPK, and c-Jun. Upstream activators of the SAPK/JNK cascade include ceramide, small GTP-binding proteins such as Rac1 and Cdc42Hs, Ask1, and caspases. MKK7 is also a major and direct SAPK/JNK activator in the TNFŒ± or environmental stress signaling pathways, where its kinase activity directly phosphorylates SAPK/JNK. This relationship between MKK7 and SAPK appears to be evolutionarily conserved, as it is preserved in their Drosophila homologues, Hep and DJNK, respectively.

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 2

Tested Applications	FC
Dilution Range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	BC33
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Thr183/Tyr185 of human phospho SAPK/JNK
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Mitogen-activated protein kinase 8, MAPK8, Stress-activated protein kinase 1c, SAPK1c, c-Jun N-terminal kinase 1, JNK1, PRKM8

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Phospho-SAPK/JNK (Thr183/Tyr185) rabbit mAb Antibody

Peptide blocking flow cytometric analysis of 293T cells secondary antibody only negative control (light blue) or treated with K252a (red) or with UV + TPA (green) or K252a and blocked with phospho-peptide (black) or UV and blocked with phospho pepeptide (gold) or K252a and blocked with non-phospho peptide (blue) or UV and blocked with non-phospho peptide (purple) using Phospho-SAPK/JNK (Thr183/Tyr185) antibody SAPKT183Y185-A11 at 0.01 μg/mL. Cat. #orb1946073.

Flow cytometric analysis of 293T cells secondary antibody only negative control (blue) or treated with K252a (red) or with UV + TPA (green) using Phospho-SAPK/JNK (Thr183/Tyr185) antibody SAPKT183Y185-A11 at 0.01 μg/mL. Cat. #orb1946073.

Quick Database Links

UniProt

P45983

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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