Phospho-Serine/Threonine Rabbit Polyclonal Antibody

SKU: orb158370

Description

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, ICC, IF, IHC-Fr, IHC-P
Dilution range	IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, ELISA=1:5000-10000
Reactivity	All
Predicted Reactivity	Other

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthesised phosphopeptide contain Phosphoserine and Phosphothreonine (p-S)(p-T)-NH2
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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phosphoserine/threonine; phospho-Ser/phospho-Thr; pan phospho-Ser/phospho-Thr; pan-phospho-Ser/phospho-Thr; pS/pT; p-S/p-T; pSER/pThr; p-SER/p-Thr; p-Sp-T; pan pS/pT; pan p-S/p-T; pan pSER/pThr; pan p-SER/p-Thr; pan p-Sp-T..

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Phospho-Serine/Threonine Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phosphoserine, threonine) Polyclonal Antibody, Unconjugated (orb158370) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phosphoserine, threonine) Polyclonal Antibody, Unconjugated (orb158370) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phosphoserine, threonine) Polyclonal Antibody, Unconjugated (orb158370) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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