Phospho-Smad3 (Ser423 + Ser425) Recombinant Rabbit Monoclonal Antibody

SKU: orb526653

Description

Research Area

Cancer Research, Cell Biology, Cell Signaling, Cytoplasmic, Hypoxia, Intracellular Signaling Proteins, SMAD Family

Images & Validation

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Item 1 of 1

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Human, Mouse
Predicted Reactivity	Porcine, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	5C5
Immunogen	KLH conjugated Synthesised phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425 CS(p-S)V(p-S)
Target	SMAD3
Molecular Weight	47 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Smad3(phospho Ser423/425); Smad3(phospho S423+S425); p-Smad3(Ser423+Ser425); hMAD 3; hSMAD3; HSPC193; JV15 2; JV152; MAD(mothers against decapentaplegic Drosophila) homolog 3; MAD3; MADH 3; MADH3; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA and MAD related protein 3; SMAD 3; SMAD; SMAD-3; SMAD3_HUMAN.

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Phospho-Smad3 (Ser423 + Ser425) Recombinant Rabbit Monoclonal Antibody

Sample: Lung (Mouse) Lysate at 40 ug, A549 (Human) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-Smad3 (Ser423 + Ser425) (orb526653) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 55 kD.

Quick Database Links

Gene Symbol

SMAD3

UniProt

P84022

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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