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Phospho-Tau (Thr181) Antibody
SKU: orb1294393
Description
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC-Fr, WB |
|---|---|
| Dilution range | Western Blot 1:500-1:1500 Immunofluorescence 1:200–1:400 Immunohistochemistry (Paraffin) 1:100 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide corresponding to residues surrounding Thr181 of human Tau protein |
| Target | Phospho-Tau (Thr181) |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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Anti-Phospho-Tau (Thr181) antibody at 1/1000 dilution, Lysates/proteins at 55 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294393 overnight at 4°C.
Anti-Phospho-Tau (Thr181) antibody at 1/1000 dilution, Lysates/proteins at 55 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294393 overnight at 4°C.
Immunofluorescent analysis. Sample: cortical neurons, Phospho-Tau (Thr181) antibody (orb1294393): 1-200, Red: MAP2 antibody, Anti-rabbit 488: 1-500, Fixed: 4% paraformaldehyde at RT for 20 min.
Immunohistochemical analysis of paraffin embedded Human tissue labeling phosphor-Tau (Thr181) with orb1294393 at 1/100.
Immunohistochemical of frozen sections. Phospho-Tau (Thr181) antibody (orb1294393): 1-200, Red: Tuj1 antibody, Anti-rabbit 488: 1-500.
Specificity and sensitivity of Phospho-Tau (Thr181) Antibody (orb1294393).
Quick Database Links
Gene Symbol
Phospho-Tau (Thr181)
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Phospho-Tau (Thr181) Antibody (orb1294393)
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