Pig Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

SKU: orb779804

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 2(BMP2).

Research Area

Musculoskeletal & Connective Tissue Research, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig BMP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig BMP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig BMP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig BMP2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Porcine
Sample Types	Serum, plasma, tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	31.25-2000 pg/mL
Sensitivity	13.1 pg/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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BMP2A ELISA Kit, BMP-2A ELISA Kit, Hemochromatosis Modifier ELISA Kit

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Pig Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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