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Pinosylvin

SKU: orb1225633

Description

Pinosylvin induces autophagy via AMPK activation. Pinosylvin is likely to act as a pro-angiogenic factor.

Images & Validation

Key Properties

CAS Number22139-77-1
MW212.248
Purity>98% (HPLC)
FormulaC14H12O2
SMILESC1=CC=C(C=C1)/C=C/C2=CC(=CC(=C2)O)O
TargetOthers
SolubilitySoluble in DMSO

Bioactivity

In Vivo
Pinosylvin (intravenous injection; 10 mg/kg) yields the plasma AUC, urine t, CL and Vd values of 5.23 ± 1.20 mgh mL-1, 13.13 ± 2.05 h, 1.84 ± 0.44 Lh-1kg-1 and 2.29 Lkg-1, respectively in male Sprague-Dawley rats, in a PK study.
In Vitro
Pinosylvin (0-100 μM; 24 hours) is cytotoxic to THP-1 and U937 cells, exhibits an IC50 value of 20-30 μM in leukemia cells, the maximal cytotoxic effect occurred at 100 μM following incubation for 24 hr. Pinosylvin (0-100 μM; 24 hours) enhances the number of annexin V+ and PI+ cells in the U937 population at 50 μM, increasesannexin V+ and PI+ cells in the THP-1 population at 100 μM. Pinosylvin (0-100 μM; 24 hours) promotesautophagy in leukemia cells by enhancing the level ofLC3-II and p62/SQSTM1 degradation in leukemia cells. Cell Viability Assay Cell line: Leukemia cells. Concentration: 0 μM; 0.1 μM; 1 μM; 10 μM; 50 μM; 100 μM Incubation time: 24 hours. Result: Was cytotoxic to leukemia cells at high concentrations. Apoptosis Analysis. Cell line: U937 and THP-1 cells. Concentration: 0 μM; 0.1 μM; 1 μM; 10 μM; 50 μM; 100 μM Incubation time: 24 hours. Result: Induced apoptosis cell number in U937 and THP-1 cells. Western blot analysis. Cell line: U937 and THP-1 cells. Concentration: 0 μM; 0.1 μM; 1 μM; 10 μM; 50 μM; 100 μM Incubation time: 24 hours. Result: Induced autophagy in leukemia cells.

Storage & Handling

StorageStorage temperature: -20°C. Stability: ≥ 2 years
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Pinosylvin (orb1225633)

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