PKC ζ rabbit pAb Antibody

SKU: orb766101

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Description

Rabbit polyclonal antibody to PKC zeta

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Reactivity	Human, Monkey, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human PKC zeta. AA range:526-575
Molecular Weight	80
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-PRKCZ antibody, anti-PKC2 antibody, anti-Protein kinase C zeta type antibody, anti-nPKC-zeta antibody

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Western Blot analysis of various cells using PKC ζ Polyclonal Antibody diluted at 1:2000.

Western Blot analysis of COS7 cells using PKC ζ Polyclonal Antibody diluted at 1:2000.

Immunofluorescence analysis of NIH/3T3 cells, using PKC zeta Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PKC zeta Antibody. The picture on the right is blocked with the synthesized peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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