PLA2G4B Antibody (Center)

SKU: orb35212

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Description

Rabbit polyclonal antibody to PLA2G4B. The protein is highly expressed in brain, heart, liver, cerebellum and pancreasa, and is a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. In addition, it is a selectively hydrolyzes glycerophospholipids in the sn-2 position with a preference for arachidonoyl phospholipids.

Research Area

Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	FC, WB
Dilution range	WB: 1:1000, FACS: 1:10-50
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	RB21321
Immunogen	Synthetic Peptide
Target	PLA2G4B
Molecular Weight	87978
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Disclaimer	For research use only

Alternative Names

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Anti-Phospholipase A2 group IVB antibody, anti-Cytosolic phospholipase A2 beta antibody, anti-cPLA2-beta, PLA2G4B antibody

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Western blot analysis of PLA2G4B Antibody (Center) in K562 cell line lysates (35 ug/lane). PLA2G4B (arrow) was detected using the purified Pab.

PLA2G4B Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Quick Database Links

Gene Symbol

PLA2G4B

UniProt

P0C869

RefSeq (Protein):NP_001185517.1, NP_005081.1, NP_001108105.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001185517.1, NP_005081.1, NP_001108105.1

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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