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PPARA Antibody
SKU: orb1927181
Description
Research Area
Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Metabolism Research
Images & Validation
−Item 1 of 5
| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:1000 |
| Reactivity | Human, Mouse |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1,κ |
| Molecular Weight | 52225 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−NR1C1, PPAR
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Flow cytometric analysis of Hela cells using PPARA Antibody (green) compared to an isotype control of mouse IgG1 (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded H. kidney section using PPARA Antibody. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using PPARA Antibody. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from Hela, Jurkat, mouse NIH/3T3 cell line (from left to right), using PPARA Antibody. Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
Fluorescent image of Hela cells stained with PPARA Antibody. Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
PPARA Antibody (orb1927181)
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