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Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 324-647 of human PPP4R1 (NP_001035847.1). |
| Target | PPP4R1 |
| Molecular Weight | Observed: 130kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−MEG1, PP4(Rmeg), PP4R1, serine/threonine-protein phosphatase 4 regulatory subunit 1, serine/threonine phosphatase 4
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Western blot analysis of extracts of various cell lines, using PPP4R1 antibody (orb1255885) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human liver using PPP4R1 antibody (orb1255885) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PPP4R1 antibody (orb1255885) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200 ug extracts of 293T cells, using 3 ug PPP4R1 antibody (orb1255885). Western blot was performed from the immunoprecipitate using PPP4R1 antibody (orb1255885) at a dilution of 1:1000.
Quick Database Links
Gene Symbol
PPP4R1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
PPP4R1 Antibody (orb1255885)
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