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PSD93/DLG2 Recombinant Rabbit Monoclonal Antibody
SKU: orb608073
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Description
Images & Validation
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| Tested Applications | FC, WB |
|---|---|
| Dilution range | WB=1:500-2000, Flow-Cyt=1:50-100 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 6D1 |
| Immunogen | KLH conjugated synthetic peptide derived from human PSD93/DLG2 |
| Target | DLG2 |
| Molecular Weight | 98 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Channel associated protein of synapse 110; Channel associated protein of synapses 110kD; Channel-associated protein of synapse-110; Chapsyn 110; Chapsyn-110; Chapsyn110; discs large homolog 2; Discs, large homolog 2(Drosophila); Disks large homolog 2; DKFZp781D1854; DKFZp781E0954; Dlg 2; dlg2; DLG2_HUMAN; Dlgh 2; Dlgh2; FLJ37266; Gm1197; MGC131811; Postsynaptic density protein PSD 93; Postsynaptic density protein PSD-93; Postsynaptic density protein PSD93; PSD 93; PSD93.
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Blank control: SH-SY5Y. Primary Antibody (green line): Rabbit Anti-PSD93 antibody (orb608073), dilution: 1:50, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1:1000. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Sample: Lane 1: Hela cell lysate, Lane 2: SH-SY5Y cell lysate, Primary: Anti-PSD93/DLG2 (orb608073) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 98 kD, Observed band size: 100 kD.
Quick Database Links
Gene Symbol
DLG2
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
PSD93/DLG2 Recombinant Rabbit Monoclonal Antibody (orb608073)
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